PROMER tech is composed of DNA-RNA-DNA structure and performs primer and probe functions simultaneously.
PROMER performs both functions of primer and probe in a single nucleic acid. As a result, PROMER requires smaller amount of oligos, reduces dimer formation, and is more cost-effective.
PROMER initiates extension only when the RNA site perfectly matches the target sequence. This feature prevents non-specific extension, and ensures superior performance in point mutation detection.
RNA site in PROMER is denatured from the template when extended. It enables error corrected amplification even in case of non-specific extension, so called template dependent amplification.
LOW SPATIAL LIMITATION
PROMER is a single nucleotide, unlike conventional methods consisting of primer and probe. PROMER is more adaptable to small amplicons such as micro RNA due to its lower spatial limitation.